L5640

Sigma

 

Lectin from Agaricus bisporus (mushroom)

lyophilized powder

Synonym:Agaricus bisporus agglutinin, ABA
MDL number:MFCD01191576

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Description

Analysis NoteAgglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol ActionsABA is a mixture of two phytohemagglutinins with similar specificities for carbohydrate receptors.1 The lectin is not blood group specific, but has an affinity for fetuin.
Preparation NotePurified by affinity chromatography.
Physical formLyophilized powder containing NaCl

Properties

formlyophilized powder
potency<16 μg protein per mL agglutination activity
compositionProtein, ~10% biuret
storage temp.−20°C

Safety

WGK Germany3

References

Cited Reference1. Presant, C.A. and Kornfeld, S. J. Biol. Chem. 247, 6937, (1972)